* The tri-supports legs must be balance correctly so that the filter is parallel to the electrodes. The ENDOHM-12 (#EVM-EL-03-01-02) is compatible with the following chambers: Corning * The tri-supports overhang the chamber edge and the well cannot be held parallel to the electrodes. The ENDOHM-6 (#EVM-EL-03-01-01) is compatible with the following chambers: Corning Endohm chambers may be sterilized with EtO, alcohol or a bactericide not autoclavable.
To date, cups from Corning, Millipore, Nunc, Greiner and BD Falcon have been tested.
Oilfilterthread evom manual#
Endohm together with EVOM™ Manual offers the most accurate and economical endothelial ohmmeter now available. Because of the uniform density of the AC square wave current from EVOM™ Manual, errors owing to electrode polarization or membrane capacitance are largely eliminated. Compared with other resistance measurement methods, Endohm with EVOM2™ offers a much more convenient and economic solution to “leaky tissue” measurement. With Endohm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30 Ω with STX2 electrodes (depending on the experience of the user) to 1-2 Ω. The background resistance of a blank insert is reduced from 150 Ω (when using WPI’s hand-held STX2 electrodes) to less than 5 Ω. Make more precise measurements with EndohmsĮndohm’s symmetrically opposing circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density to flow across the membrane than with the basic STX2 electrodes. The height of the top electrode can be adjusted to fit cell culture cups of different manufacture.
Oilfilterthread evom plus#
The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. Using WPI’s EVOM™ Manual resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial and epithelial monolayers in culture cups. TEER measurement for removable culture cup systems using EVOM™ Manual meters for endothelial and epithelial cell cultures.Simple test procedure to verify electrode performance.Tri-leg supports offer mechanical stability and the membrane is held parallel to the electrodes (G version).Symmetrical electrode pattern disperses test current uniformly.
Can be used with 6, 12 or 24 well plates with removable inserts.Stability and reproducibility superior to the STX2 electrodes to 1% tolerance.ENDOHM-6 EVOM™ Electrode for TEER in 6.5 mm InsertĮNDOHM-12 EVOM™ Electrode for TEER in 12 mm InsertĮNDOHM-24 EVOM™ Electrode for TEER in 24 mm Insert
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